Method Matters: Effect of Purification Technology on Neutrophil Phenotype and Function.

ABSTRACT: Neutrophils are the most abundant leukocytes in human blood and the first cells responding to infection and injury. Due to their limited ex vivo lifespan and the impossibility to cryopreserve or expand them in vitro, neutrophils need to be purified from fresh blood for immediate use in experiments. Importantly, neutrophil purification methods may artificially modify the phenotype and functional characteristics of the isolated cells. The aim of this study was to expose the effects of 'classical' density-gradient purification versus the more expensive but faster immunomagnetic isolation on neutrophil phenotype and functionality. We found that in the absence of inflammatory stimuli, density-gradient-derived neutrophils showed increased polarization responses as well as en

SUBMITTER: Blanter M 

PROVIDER: S-EPMC8866851 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature