FIONA1-Mediated m⁶ A Modification Regulates the Floral Transition in Arabidopsis.

ABSTRACT: N⁶ -methyladenosine (m⁶ A) mRNA modification represents the most widespread form of internal modifications in eukaryotic mRNAs. In the model plant Arabidopsis thaliana, those known methyltransferases mainly deposit m⁶ A at their target transcripts near the stop codon or in the 3' untranslated region. Here, it is reported that FIONA1 (FIO1), a human METTL16 ortholog, acts as a hitherto unknown m⁶ A methyltransferase that determines m⁶ A modifications at over 2000 Arabidopsis transcripts predominantly in the coding region. Mutants of FIO1 show a decrease in global m⁶ A mRNA methylation levels and an early-flowering phenotype. Nanopore direct RNA sequencing reveals that FIO1 is required for establishing appropriate levels of m<

SUBMITTER: Xu T 

PROVIDER: S-EPMC8867147 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature