Acceleration of Bone Regeneration Induced by a Soft-Callus Mimetic Material.

ABSTRACT: Clinical implementation of endochondral bone regeneration (EBR) would benefit from the engineering of devitalized cartilaginous constructs of allogeneic origins. Nevertheless, development of effective devitalization strategies that preserves extracellular matrix (ECM) is still challenging. The aim of this study is to investigate EBR induced by devitalized, soft callus-mimetic spheroids. To challenge the translatability of this approach, the constructs are generated using an allogeneic cell source. Neo-bone formation is evaluated in an immunocompetent rat model, subcutaneously and in a critical size femur defect. Living spheroids are used as controls. Also, the effect of spheroid maturation towards hypertrophy is evaluated. The devitalization procedure successfully induces cell death withou

SUBMITTER: Longoni A 

PROVIDER: S-EPMC8867155 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature