Selective radiosensitization by nitazoxanide of quiescent clonogenic colon cancer tumour cells.

ABSTRACT: Nitazoxanide is a Food and Drug Administration-approved antiprotozoal drug recently demonstrated to be selectively active against quiescent and glucose-deprived tumour cells. This drug also has several characteristics that suggest its potential as a radiosensitizer. The present study aimed to investigate the interaction between nitazoxanide and radiation on human colon cancer cells cultured as monolayers, and to mimic key features of solid tumours in patients, as spheroids, as well as in xenografts in mice. In the present study, colon cancer HCT116 green fluorescent protein (GFP) cells were exposed to nitazoxanide, radiation or their combination. Cell survival was analysed by using total cell kill and clonogenic assays. DNA double-strand breaks were evaluated in the spheroid experiments, a

SUBMITTER: Karlsson H 

PROVIDER: S-EPMC8867181 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature