Simultaneous Determination of Vitamin D and Its Hydroxylated and Esterified Metabolites by Ultrahigh-Performance Supercritical Fluid Chromatography-Tandem Mass Spectrometry.

ABSTRACT: In this study, an analytical method has been developed that, for the first time, allows simultaneous determination of vitamin D₂ and vitamin D₃ along with their hydroxylated and esterified forms. A group of 12 vitamin D analogues including vitamin D₂ and vitamin D₃, seven hydroxylated metabolites, and three ester forms were separated in a single 8.0 min run using ultrahigh-performance supercritical fluid chromatography coupled with triple quadrupole tandem mass spectrometry. Electrospray ionization and atmospheric pressure chemical ionization were investigated as ion sources, of which the latter showed a higher ionization efficiency. Chromatographic conditions were thoroughly evaluated by a step-by-step method, whereas an experimental design was

SUBMITTER: Socas-Rodriguez B 

PROVIDER: S-EPMC8867463 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature