Combined use of Oxford Nanopore and Illumina sequencing yields insights into soybean structural variation biology.

ABSTRACT:

Background

Structural variants (SVs), including deletions, insertions, duplications, and inversions, are relatively long genomic variations implicated in a diverse range of processes from human disease to ecology and evolution. Given their complex signatures, tendency to occur in repeated regions, and large size, discovering SVs based on short reads is challenging compared to single-nucleotide variants. The increasing availability of long-read technologies has greatly facilitated SV discovery; however, these technologies remain too costly to apply routinely to population-level studies. Here, we combined short-read and long-read sequencing technologies to provide a comprehensive population-scale assessment of structural variation in a panel of Canadian soybean cultivars.

Results<

SUBMITTER: Lemay MA 

PROVIDER: S-EPMC8867729 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature