YTHDF1 promotes breast cancer progression by facilitating FOXM1 translation in an m6A-dependent manner.

ABSTRACT:

Background

N6-methyladenosine (m⁶A) is the most common post-transcriptional modification at the RNA level. However, the exact molecular mechanisms of m6A epigenetic regulation in breast cancer remain largely unknown and need to be fully elucidated.

Methods

 The integrating bioinformatics analyses were used to screen clinical relevance and dysregulated m6A "reader" protein YTHDF1 in breast cancer from TCGA databases, which was further validated in a cohort of clinical specimens. Furthermore, functional experiments such as the CCK-8 assay, EdU assay, wound healing assay, transwell invasion assay and cell cycle assay were used to determine the biological role of YTHDF1 in breast cancer. RIP, m6A-IP, and CLIP assays were used to find the target of YTHDF1 and further v

SUBMITTER: Chen H 

PROVIDER: S-EPMC8867832 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature