Dual-Channel Electrochemical Measurements Reveal Rapid Adenosine is Localized in Brain Slices.

ABSTRACT: Rapid adenosine signaling has been detected spontaneously or after mechanical stimulation in the brain, providing rapid neuromodulation in a local area. To measure rapid adenosine signaling, a single carbon-fiber microelectrode has traditionally been used, which limits spatial resolution and an understanding of regional coordination. In this study, we utilized dual-channel fast-scan cyclic voltammetry to measure the spontaneous or mechanically stimulated adenosine release at two electrodes placed at different spacings in hippocampal CA1 mouse brain slices. For mechanically stimulated adenosine release, adenosine can be detected up to 150 μm away from where it was stimulated, although the signal is smaller and delayed. While spontaneous adenosine transients were detected at both electrodes,

SUBMITTER: Chang Y 

PROVIDER: S-EPMC8867919 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature