Frequency, composition and mobility of Escherichia coli-derived transposable elements in holdings of plasmid repositories.

ABSTRACT: By providing the scientific community with uniform and standardized resources of consistent quality, plasmid repositories play an important role in enabling scientific reproducibility. Plasmids containing insertion sequence elements (IS elements) represent a challenge from this perspective, as they can change the plasmid structure and function. In this study, we conducted a systematic analysis of a subset of plasmid stocks distributed by plasmid repositories (The Arabidopsis Biological Resource Center and Addgene) which carry unintended integrations of bacterial mobile genetic elements. The integration of insertion sequences was most often found in, but not limited to, pBR322-derived vectors, and did not affect the function of the specific plasmids. In certain cases, the entire stock was a

SUBMITTER: Brkljacic J 

PROVIDER: S-EPMC8867978 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature