Enhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering.

ABSTRACT: Edeines, a group of cationic antimicrobial peptides produced by the soil bacterium Brevibacillus, have broad biological effects, such as antimicrobial, anticancer and immunosuppressive activities. However, the yield of edeines in wild-type (WT) Brevibacillus is extremely low, and chemical synthesis of edeines is a time-consuming process. Genetic engineering has proven to be an effective approach to produce antibiotics with high yield. In this study, the edeine biosynthetic gene cluster (ede BGC), which is involved in edeine production, was identified and characterized in Brevibacillus brevis X23. To improve edeine production in B. brevis X23, the ede BGC promoter was replaced with six different promoters, P_mwp , P_spc , P_xylA , P_shuttle-09 , P

SUBMITTER: Liu Q 

PROVIDER: S-EPMC8867987 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature