Mitochondrial Function Differences between Tumor Tissue of Human Metastatic and Premetastatic CRC.

ABSTRACT: Most colorectal cancer (CRC) patients die as a consequence of metastasis. Mitochondrial dysfunction could enhance cancer development and metastatic progression. We aimed to evaluate the adaptations associated with mitochondrial function in tumor tissues from stages III and IV of human CRC and whether they could ultimately be used as a therapeutic target in metastatic colorectal cancer (mCRC). We analyzed the protein levels by Western blotting and the enzymatic activities of proteins involved in mitochondrial function, as well as the amount of mitochondrial DNA (mtDNA), by real-time PCR, analyzing samples of non-tumor adjacent tissue and tumor tissue from stages III and IV CRC patients without radio- or chemotherapy treatment prior to surgery. Our data indicate that the tumor tissue of pre-

SUBMITTER: Hernandez-Lopez R 

PROVIDER: S-EPMC8869310 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature