Optimization of an Inclusion Body-Based Production of the Influenza Virus Neuraminidase in Escherichia coli.

ABSTRACT: Neuraminidase (NA), as an important protein of influenza virus, represents a promising target for the development of new antiviral agents for the treatment and prevention of influenza A and B. Bacterial host strain Escherichia coli BL21 (DE3)pLysS containing the NA gene of the H1N1 influenza virus produced this overexpressed enzyme in the insoluble fraction of cells in the form of inclusion bodies. The aim of this work was to investigate the effect of independent variables (propagation time, isopropyl β-d-1-thiogalactopyranoside (IPTG) concentration and expression time) on NA accumulation in inclusion bodies and to optimize these conditions by response surface methodology (RSM). The maximum yield of NA (112.97 ± 2.82 U/g) was achieved under optimal conditions, namely, a propa

SUBMITTER: Lipnicanova S 

PROVIDER: S-EPMC8869668 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature