Generation and Characterization of an Inducible Cx43 Overexpression System in Mouse Embryonic Stem Cells.

ABSTRACT: Connexins (Cx) are a large family of membrane proteins that can form intercellular connections, so-called gap junctions between adjacent cells. Cx43 is widely expressed in mammals and has a variety of different functions, such as the propagation of electrical conduction in the cardiac ventricle. Despite Cx43 knockout models, many questions regarding the biology of Cx43 in health and disease remain unanswered. Herein we report the establishment of a Cre-inducible Cx43 overexpression system in murine embryonic stem (ES) cells. This enables the investigation of the impact of Cx43 overexpression in somatic cells. We utilized a double reporter system to label Cx43-overexpressing cells via mCherry fluorescence and exogenous Cx43 via fusion with P2A peptide to visualize its distribution pattern.

SUBMITTER: Niemann P 

PROVIDER: S-EPMC8869955 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature