Divalent Metal Ions Boost Effect of Nucleic Acids Delivered by Cell-Penetrating Peptides.

ABSTRACT: Cell-penetrating peptides (CPPs) are promising tools for the transfection of various substances, including nucleic acids, into cells. The aim of the current work was to search for novel safe and effective approaches for enhancing transfection efficiency of nanoparticles formed from CPP and splice-correcting oligonucleotide (SCO) without increasing the concentration of peptide. We analyzed the effect of inclusion of calcium and magnesium ions into nanoparticles on CPP-mediated transfection in cell culture. We also studied the mechanism of such transfection as well as its efficiency, applicability in case of different cell lines, nucleic acid types and peptides, and possible limitations. We discovered a strong positive effect of these ions on transfection efficiency of SCO, that translated t

SUBMITTER: Maloverjan M 

PROVIDER: S-EPMC8870069 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature