Synergistic Cytokine Production by ATP and PGE₂ via P2X4 and EP₃ Receptors in Mouse Bone-Marrow-Derived Mast Cells.

ABSTRACT: ATP is an important intercellular messenger in the extracellular space. In mast cells (MCs), ATP stimulates the ionotropic P2X4 receptor (P2X4R), resulting in enhanced degranulation and exacerbation of acute allergic reactions. In this study, we investigate whether ATP regulates inflammatory cytokine production in MCs. Gene expression was analyzed by quantitative RT-PCR, and cytokine production was measured using ELISA. The stimulation of mouse bone-marrow-derived MCs (BMMCs) with ATP alone had little effect on cytokine secretion. However, the co-stimulation with prostaglandin (PG) E₂ resulted in a marked increase in the secretion of various cytokines, such as tumor necrosis factor-α, interleukin (IL)-6, and IL-13, accompanied by an increase in their mRNA levels. The effects of

SUBMITTER: Obayashi K 

PROVIDER: S-EPMC8870111 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature