Nickel (II) and Cobalt (II) Alginate Biopolymers as a "Carry and Release" Platform for Polyhistidine-Tagged Proteins.

ABSTRACT: Protein immobilization using biopolymer scaffolds generally involves undesired protein loss of function due to denaturation, steric hindrance or improper orientation. Moreover, most methods for protein immobilization require expensive reagents and laborious procedures. This work presents the synthesis and proof of concept application of two alginate hydrogels that are able to bind proteins with polyhistidine tags by means of interaction with the crosslinking cations. Nickel (II) and cobalt (II) alginate hydrogels were prepared using a simple ionic gelation method. Hydrogels were characterized by optical microscopy and AFM, and evaluated for potential cytotoxicity. In addition, binding capacity was tested towards proteins with or without HisTAG. Hydrogels had moderate cytotoxicity and were

SUBMITTER: Dumitrascu AM 

PROVIDER: S-EPMC8871198 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature