Modifying the m⁶A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic tagging.

ABSTRACT: Synaptic plasticity processes, which underlie learning and memory formation, require RNA to be translated local to synapses. The synaptic tagging hypothesis has previously been proposed to explain how mRNAs are available at specific activated synapses. However how RNA is regulated, and which transcripts are silenced or processed as part of the tagging process is still unknown. Modification of RNA by N6-methyladenosine (m⁶A/m) influences the cellular fate of mRNA. Here, by advanced microscopy, we showed that m⁶A demethylation by the eraser protein ALKBH5 occurs at active synaptic ribosomes and at synapses during short term plasticity. We demonstrated that at activated glutamatergic post-synaptic sites, both the YTHDF1 and YTHDF3 reader and the ALKBH5 eraser proteins in

SUBMITTER: Martinez De La Cruz B 

PROVIDER: S-EPMC8872986 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature