New transgenic mouse models enabling pan-hematopoietic or selective hematopoietic stem cell depletion in vivo.

ABSTRACT: Hematopoietic stem cell (HSC) multipotency and self-renewal are typically defined through serial transplantation experiments. Host conditioning is necessary for robust HSC engraftment, likely by reducing immune-mediated rejection and by clearing limited HSC niche space. Because irradiation of the recipient mouse is non-specific and broadly damaging, there is a need to develop alternative models to study HSC performance at steady-state and in the absence of radiation-induced stress. We have generated and characterized two new mouse models where either all hematopoietic cells or only HSCs can be specifically induced to die in vivo or in vitro. Hematopoietic-specific Vav1-mediated expression of a loxP-flanked diphtheria-toxin receptor (DTR) renders all hematopoietic cells sensitive to diphthe

SUBMITTER: Rodriguez Y Baena A 

PROVIDER: S-EPMC8873235 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature