Exosomes Mediate APP Dysregulation via APP-miR-185-5p Axis.

ABSTRACT: APP misexpression plays a crucial role in triggering a complex pathological cascade, leading to Alzheimer's disease (AD). But how the expression of APP is regulated in pathological conditions remains poorly understood. In this study, we found that the exosomes isolated from AD mouse brain promoted APP expression in neuronal N2a cells. Moreover, exosomes derived from N2a cells with ectopic expression of APP (APP-EXO) also induced APP dysregulation in normal N2a cells. Surprisingly, the effects of APP-EXO on APP expression in recipient cells were not mediated by the direct transferring of APP gene products. Instead, the effects of APP-EXO were highly likely mediated by the reduction of the expression levels of exosomal miR-185-5p. We found that the 3'UTR of APP transcripts bind

SUBMITTER: Ding L 

PROVIDER: S-EPMC8873530 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature