Desmoplakin Maintains Transcellular Keratin Scaffolding and Protects From Intestinal Injury.

ABSTRACT:

Background & aims

Desmosomes are intercellular junctions connecting keratin intermediate filaments of neighboring cells. The cadherins desmoglein 2 (Dsg2) and desmocollin 2 mediate cell-cell adhesion, whereas desmoplakin (Dsp) provides the attachment of desmosomes to keratins. Although the importance of the desmosome-keratin network is well established in mechanically challenged tissues, we aimed to assess the currently understudied function of desmosomal proteins in intestinal epithelia.

Methods

We analyzed the intestine-specific villin-Cre DSP (DSP^ΔIEC) and the combined intestine-specific DSG2/DSP^ΔIEC (ΔDsg2/Dsp) knockout mice. Cross-breeding with keratin 8-yellow fluorescent protein knock-in mice and generation of organoids was performed to visualize

SUBMITTER: Gross A 

PROVIDER: S-EPMC8873596 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature