Hepatic Reduction in Cholesterol 25-Hydroxylase Aggravates Diet-induced Steatosis.

ABSTRACT:

Background & aims

Cholesterol 25-hydroxylase (Ch25h), converting cholesterol to 25-hydroxycholesterol (25-HC), is critical in modulating cellular lipid metabolism and anti-inflammatory and antiviral activities. However, its role in nonalcoholic fatty liver disease remains unclear.

Methods

Ch25h expression was detected in livers of ob/ob mice and E3 rats fed a high-fat diet (HFD). Gain- or loss-of-function of Ch25h was performed using Ch25h^+/+ (wild type [WT]) mice receiving AAV8-Ch25h or Ch25h knockout (Ch25h^-/-) mice. WT mice fed an HFD were administered with 25-HC. The Ch25h-LXRα-CYP axis was measured in primary hepatocytes isolated from WT and Ch25h^-/- mice.

Results

We found that Ch25h level was decreased in livers of ob/ob mice and

SUBMITTER: Dong Z 

PROVIDER: S-EPMC8873960 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature