An Efficient Genetic Transformation and CRISPR/Cas9-Based Genome Editing System for Moso Bamboo (Phyllostachys edulis).

ABSTRACT: Moso bamboo (Phyllostachys edulis) is the most important monopodial bamboo species worldwide. Without a genetic transformation system, it is difficult to verify the functions of genes controlling important traits and conduct molecular breeding in moso bamboo. Here, we established a plant regeneration system from immature embryos. Calli were induced on MS medium added 4-6 mg⋅L^-1 2,4-dichlorophenoxyacetic acid (2,4-D) with high efficiency (>60%). A plant growth regulator combination of 0.5 mg⋅L^-1 1-naphthylacetic acid (NAA), 2.0 mg⋅L^-1 6-benzylaminopurine (BAP), and 3.0 mg⋅L^-1 zeatin (ZT) was suitable for shoot differentiation, and the shoot induction frequency was increased to 43% after 0.5 mg⋅L^-1 abscisic acid (ABA) pretreatment

SUBMITTER: Huang B 

PROVIDER: S-EPMC8874139 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature