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Metabolic Engineering of Escherichia coli for Methyl Parathion Degradation.


ABSTRACT: Organophosphate compounds are widely used in pesticides to control weeds, crop diseases, and insect pests. Unfortunately, these synthetic compounds are hazardous and toxic to all types of living organisms. In the present work, Escherichia coli was bioengineered to achieve methyl parathion (MP) degradation via the introduction of six synthetic genes, namely, opdS, pnpAS, pnpBS, pnpCS, pnpDS, and pnpES, to obtain a new transformant, BL-MP. MP and its subsequent decomposition intermediates were completely degraded by this transformant to enter the metabolites of multiple anabolic pathways. The MP-degraded strain created in this study may be a promising candidate for the bioremediation of MP and potential toxic intermediates.

SUBMITTER: Xu J 

PROVIDER: S-EPMC8874220 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Metabolic Engineering of <i>Escherichia coli</i> for Methyl Parathion Degradation.

Xu Jing J   Wang Bo B   Wang Ming-Qing MQ   Gao Jian-Jie JJ   Li Zhen-Jun ZJ   Tian Yong-Sheng YS   Peng Ri-He RH   Yao Quan-Hong QH  

Frontiers in microbiology 20220211


Organophosphate compounds are widely used in pesticides to control weeds, crop diseases, and insect pests. Unfortunately, these synthetic compounds are hazardous and toxic to all types of living organisms. In the present work, <i>Escherichia coli</i> was bioengineered to achieve methyl parathion (MP) degradation via the introduction of six synthetic genes, namely, <i>opdS</i>, <i>pnpAS</i>, <i>pnpBS</i>, <i>pnpCS</i>, <i>pnpDS</i>, and <i>pnpES</i>, to obtain a new transformant, BL-MP. MP and it  ...[more]

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