Sodium Energetic Cycle in the Natronophilic Bacterium Thioalkalivibrio versutus.

ABSTRACT: As inhabitants of soda lakes, Thioalkalivibrio versutus are halo- and alkaliphilic bacteria that have previously been shown to respire with the first demonstrated Na⁺-translocating cytochrome-c oxidase (CO). The enzyme generates a sodium-motive force (Δs) as high as -270 mV across the bacterial plasma membrane. However, in these bacteria, operation of the possible Δs consumers has not been proven. We obtained motile cells and used them to study the supposed Na⁺ energetic cycle in these bacteria. The resulting motility was activated in the presence of the protonophore 2-heptyl-4-hydroxyquinoline N-oxide (HQNO), in line with the same effect on cell respiration, and was fully blocked by amiloride-an inhibitor of Na⁺-motive flagella.

SUBMITTER: Muntyan MS 

PROVIDER: S-EPMC8874543 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature