Computational Design of Single-Peptide Nanocages with Nanoparticle Templating.

ABSTRACT: Protein complexes perform a diversity of functions in natural biological systems. While computational protein design has enabled the development of symmetric protein complexes with spherical shapes and hollow interiors, the individual subunits often comprise large proteins. Peptides have also been applied to self-assembly, and it is of interest to explore such short sequences as building blocks of large, designed complexes. Coiled-coil peptides are promising subunits as they have a symmetric structure that can undergo further assembly. Here, an α-helical 29-residue peptide that forms a tetrameric coiled coil was computationally designed to assemble into a spherical cage that is approximately 9 nm in diameter and presents an interior cavity. The assembly comprises 48 copies of the designed

SUBMITTER: Villegas JA 

PROVIDER: S-EPMC8874777 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature