Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses.

ABSTRACT: Small ruminant lentiviruses (SRLVs), i.e., CAEV and MVV, cause insidious infections with life-long persistence and a slowly progressive disease, impairing both animal welfare and productivity in affected herds. The complex diagnosis of SRLVs currently combines serological methods including whole-virus and peptide-based ELISAs and Immunoblot. To improve the current diagnostic protocol, we analyzed 290 sera of animals originating from different European countries in parallel with three commercial screening ELISAs, Immunoblot as a confirmatory assay and five SU5 peptide ELISAs for genotype differentiation. A newly developed nested real-time PCR was carried out for the detection and genotype differentiation of the virus. Using a heat-map display of the combined results, the drawbacks of the cu

SUBMITTER: Schaer J 

PROVIDER: S-EPMC8875174 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature