Development and Validation of a New TaqMan Real-Time PCR for the Detection of Ornithobacterium rhinotracheale.

ABSTRACT: Ornithobacterium rhinotracheale (ORT) has been associated with poultry respiratory disease worldwide. The organism is fastidious and isolation is challenging. One TaqMan real-time PCR (qPCR) assay has been developed for the detection of ORT. However, during validating the ORT qPCR, the assay performance was suboptimal. During the in silico evaluation, deviations from the basic parameters for primers and probes designs (e.g., presence of stable undesirable primer-dimers) were observed. The suboptimal design led to low efficiency and low sensitivity of the assay. Initially, modification on the probe was carried out to improve the performance of the assay. However, the assay's performance (efficiency and sensitivity) was still suboptimal. In this manuscript, we describe the development

SUBMITTER: Hashish A 

PROVIDER: S-EPMC8875355 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature