Systematic Evaluation of HILIC Stationary Phases for Global Metabolomics of Human Plasma.

ABSTRACT: Polar hydrophilic metabolites have been identified as important actors in many biochemical pathways. Despite continuous improvement and refinement of hydrophilic interaction liquid chromatography (HILIC) platforms, its application in global polar metabolomics has been underutilized. In this study, we aimed to systematically evaluate polar stationary phases for untargeted metabolomics by using HILIC columns (neutral and zwitterionic) that have been exploited widely in targeted approaches. To do so, high-resolution mass spectrometry was applied to thoroughly investigate selectivity, repeatability and matrix effect at three pH conditions for 9 classes of polar compounds using 54 authentic standards and plasma matrix. The column performance for utilization in untargeted metabolomics was assess

SUBMITTER: Hosseinkhani F 

PROVIDER: S-EPMC8875576 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature