Oocyte Penetration Speed Optimization Based on Intracellular Strain.

ABSTRACT: Oocyte penetration is an essential step for many biological technologies, such as animal cloning, embryo microinjection, and intracytoplasmic sperm injection (ICSI). Although the success rate of robotic cell penetration is very high now, the development potential of oocytes after penetration has not been significantly improved compared with manual operation. In this paper, we optimized the oocyte penetration speed based on the intracellular strain. We firstly analyzed the intracellular strain at different penetration speeds and performed the penetration experiments on porcine oocytes. Secondly, we studied the cell development potential after penetration at different penetration speeds. The statistical results showed that the percentage of large intracellular strain decreased by 80% and the

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8875814 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature