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Prevalence and Characterization of PVL-Positive Staphylococcus aureus Isolated from Raw Cow's Milk.


ABSTRACT: The present study aimed to investigate the prevalence, antibiotic susceptibility profiles, and some toxin genes of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus (S. aureus) in unpasteurized raw cow's milk collected from retail outlets located at Mansoura, Dakahliya governorate, Egypt. In that context, a total of 700 raw cow's milk samples were investigated for the presence of S. aureus, which was identified in 41.1% (288/700) of the samples. Among the S. aureus isolates, 113 PVL-positive S. aureus were identified and subjected for further analysis. The PVL-positive S. aureus were investigated for the existence of toxin-related genes, including hemolysin (hla), toxic shock syndrome toxin-1 (tst), and enterotoxins (sea, seb, sec, see, seg, sei, and selj). Genotypic resistance of PVL-positive strains was performed for the detection of blaZ and mecA genes. Among the PVL-positive S. aureus, sea, seb, and sec were detected in 44.2, 6.2%, and 0.9%, respectively, while the hla and tst genes were identified in 54.9% and 0.9%, respectively. The blaZ and mecA genes were successfully identified in 84.9 (96/113) and 32.7% (37/113) of the total evaluated S. aureus isolates, respectively. PVL-positive S. aureus displayed a high level of resistance to penicillin, ampicillin, and trimethoprim-sulfamethoxazole. Multidrug resistance (resistant to ≥3 antimicrobial classes) was displayed by all methicillin-resistant S. aureus (MRSA) and 38.2% of methicillin-sensitive S. aureus (MSSA) isolates. The obtained findings are raising the alarm of virulent PVL-positive MRSA clones in retail milk in Egypt, suggesting the requirement for limiting the use of β-lactam drugs in food-producing animals and the importance of implementing strong hygiene procedures in dairy farms and processing plants.

SUBMITTER: Sadat A 

PROVIDER: S-EPMC8876356 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Prevalence and Characterization of PVL-Positive <i>Staphylococcus aureus</i> Isolated from Raw Cow's Milk.

Sadat Asmaa A   Shata Radwa Reda RR   Farag Alshimaa M M AMM   Ramadan Hazem H   Alkhedaide Adel A   Soliman Mohamed Mohamed MM   Elbadawy Mohamed M   Abugomaa Amira A   Awad Amal A  

Toxins 20220125 2


The present study aimed to investigate the prevalence, antibiotic susceptibility profiles, and some toxin genes of Panton-Valentine leukocidin (PVL)-positive <i>Staphylococcus aureus</i> (<i>S. aureus</i>) in unpasteurized raw cow's milk collected from retail outlets located at Mansoura, Dakahliya governorate, Egypt. In that context, a total of 700 raw cow's milk samples were investigated for the presence of <i>S. aureus</i>, which was identified in 41.1% (288/700) of the samples. Among the <i>S  ...[more]

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