Engineered Fungus Thermothelomyces thermophilus Producing Plant Storage Proteins.

ABSTRACT: An efficient Agrobacterium-mediated genetic transformation based on the plant binary vector pPZP-RCS2 was carried out for the multiple heterologous protein production in filamentous fungus Thermothelomyces thermophilus F-859 (formerly Myceliophthora thermophila F-859). The engineered fungus Th. thermophilus was able to produce plant storage proteins of Zea mays (α-zein Z19) and Amaranthus hypochondriacus (albumin A1) to enrich fungal biomass by valuable nutritional proteins and improved amino acid content. The mRNA levels of z19 and a1 genes were significantly dependent on their driving promoters: the promoter of tryptophan synthase (PtrpC) was more efficient to expres

SUBMITTER: Balabanova L 

PROVIDER: S-EPMC8877005 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature