Molecular Characterization and Pathogenicity of a Novel Soybean-Infecting Monopartite Geminivirus in China.

ABSTRACT: Soybean is a major legume crop that plays an important role in food production, industrial production, and animal husbandry. Here, we characterize a novel soybean-infecting monopartite geminivirus identified in China. Analysis of the contigs de novo assembled from sequenced small interfering RNAs, followed by PCR, cloning, and sequencing, the complete viral genome was determined to be 2782 nucleotides. The genome contains the conserved nonanucleotide sequence, TAATATTAC and other sequence features typical of the family Geminiviridae, and encodes two and four open reading frames in the virion-sense and the complementary-sense strands, respectively. Genome-wide pairwise identity analysis revealed that the novel virus shares less than 65.6% identity with previously characterized

SUBMITTER: Du M 

PROVIDER: S-EPMC8877103 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature