Preparation and Functional Identification of a Novel Conotoxin QcMNCL-XIII0.1 from Conus quercinus.

ABSTRACT: Conotoxins are tools used by marine Conus snails to hunt and are a significant repository for marine drug research. Conotoxins highly selectively coordinate different subtypes of various ion channels, and a few have been used in pain management. Although more than 8000 conotoxin genes have been found, the biological activity and function of most have not yet been examined. In this report, we selected the toxin gene QcMNCL-XIII0.1 from our previous investigation and studied it in vitro. First, we successfully prepared active recombinant QcMNCL-XIII0.1 using a TrxA (Thioredoxin A)-assisted folding expression vector based on genetic engineering technology. Animal experiments showed that the recombinant QcMNCL-XIII0.1 exhibited nerve conduction inhibition similar to that of pethidine hy

SUBMITTER: Zhang H 

PROVIDER: S-EPMC8877388 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature