A New Versatile Platform for Assessment of Improved Cardiac Performance in Human-Engineered Heart Tissues.

ABSTRACT: Cardiomyocytes derived from human pluripotent stem cells (hPSC-CMs) hold a great potential as human in vitro models for studying heart disease and for drug safety screening. Nevertheless, their associated immaturity relative to the adult myocardium limits their utility in cardiac research. In this study, we describe the development of a platform for generating three-dimensional engineered heart tissues (EHTs) from hPSC-CMs for the measurement of force while under mechanical and electrical stimulation. The modular and versatile EHT platform presented here allows for the formation of three tissues per well in a 12-well plate format, resulting in 36 tissues per plate. We compared the functional performance of EHTs and their histology in three different media and demonstrated that tissues cult

SUBMITTER: Ribeiro MC 

PROVIDER: S-EPMC8877418 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature