Cooperative Chikungunya Virus Membrane Fusion and Its Substoichiometric Inhibition by CHK-152 Antibody.
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ABSTRACT:
SUBMITTER: Blijleven JS
PROVIDER: S-EPMC8877538 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Publications
Cooperative Chikungunya Virus Membrane Fusion and Its Substoichiometric Inhibition by CHK-152 Antibody.
Blijleven Jelle S JS Bouma Ellen M EM van Duijl-Richter Mareike K S MKS Smit Jolanda M JM van Oijen Antoine M AM
Viruses 20220128 2
Chikungunya virus (CHIKV) presents a major burden on healthcare systems worldwide, but specific treatment remains unavailable. Attachment and fusion of CHIKV to the host cell membrane is mediated by the E1/E2 protein spikes. We used an in vitro single-particle fusion assay to study the effect of the potent, neutralizing antibody CHK-152 on CHIKV binding and fusion. We find that CHK-152 shields the virions, inhibiting interaction with the target membrane and inhibiting fusion. The analysis of the ...[more]
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