Protease-Based Subunit Vaccine in Mice Boosts BCG Protection against Mycobacterium tuberculosis.

ABSTRACT: The significant number of people with latent and active tuberculosis infection requires further efforts to develop new vaccines or improve the Bacillus Calmette-Guérin (BCG), which is the only approved vaccine against this disease. In this study, we developed a recombinant fusion protein (PEPf) containing high-density immunodominant epitope sequences from Rv0125, Rv2467, and Rv2672 Mycobacterium tuberculosis (Mtb) proteases that proved immunogenic and used it to develop a recombinant BCG vaccine expressing the fusion protein. After challenging using Mtb, a specific immune response was recalled, resulting in a reduced lung bacterial load with similar protective capabilities to BCG. Thus BCG PEPf failed to increase the protection conferred by BCG. The PEPf was combined with Advax4 adjuvant a

SUBMITTER: Junqueira-Kipnis AP 

PROVIDER: S-EPMC8877678 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature