Interdependent Transcription of a Natural Sense/Antisense Transcripts Pair (SLC34A1/PFN3).

ABSTRACT: Natural antisense transcripts (NATs) constitute a significant group of regulatory, long noncoding RNAs. They are prominently expressed in testis but are also detectable in other organs. NATs are transcribed at low levels and co-expressed with related protein coding sense transcripts. Nowadays NATs are generally considered as regulatory, long noncoding RNAs without closer focus on the inevitable interference between sense and antisense expression. This work describes a cellular system where sense and antisense transcription of a specific locus (SLC34A1/PFN3) is induced using epigenetic modifiers and CRISPR-Cas9. The renal cell lines HEK293 and HKC-8 do not express SLC34A1/PFN3 under normal culture conditions. Five-day exposure to dexamethasone significantly stimulates sense transcript (SLC3

SUBMITTER: Zinad HS 

PROVIDER: S-EPMC8877773 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature