Enhancement of Binding Kinetics on Affinity Substrates Using Asymmetric Electroosmotic Flow on a Sinusoidal Bipolar Electrode.

ABSTRACT: In the context of the COVID-19 epidemic, enhancing the transport of analyte to a sensor surface is crucial for rapid detection of biomolecules since common conditions, including low diffusion coefficients, cause inordinately long detection times. Integrated microfluidic immunoassay chips are receiving increasing attention for their low sample volume and fast response time. We herein take advantage of asymmetric ICEO flow at a bipolar sinusoidal electrode to improve the rate of antibody binding to the reaction surface based on finite element modeling. Three different microfluidic cavities are proposed by changing the positions of the surface reaction area. We further investigate the relationship between binding enhancement and reaction surface positions, Damkohler number, and the voltage an

SUBMITTER: Wu Y 

PROVIDER: S-EPMC8878551 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature