Theranostic Microbubbles with Homogeneous Ligand Distribution for Higher Binding Efficacy.

ABSTRACT: Phospholipid-coated targeted microbubbles are used for ultrasound molecular imaging and locally enhanced drug delivery, with the binding efficacy being an important trait. The use of organic solvent in microbubble production makes the difference between a heterogeneous or homogeneous ligand distribution. This study demonstrates the effect of ligand distribution on the binding efficacy of phospholipid-coated ανβ3-targeted microbubbles in vitro using a monolayer of human umbilical-vein endothelial cells and in vivo using chicken embryos. Microbubbles with a homogeneous ligand distribution had a higher binding efficacy than those with a heterogeneous ligand distribution both in vitro and in vivo. In vitro, 1.55× more microbubbles with a homogeneous ligand distribution bound under static condi

SUBMITTER: Langeveld SAG 

PROVIDER: S-EPMC8878664 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature