Screening of TNFR1 Binding Peptides from Deinagkistrodon acutus Venom through Phage Display.

ABSTRACT: The venomous species Deinagkistrodon acutus has been used as anti-inflammatory medicine in China for a long time. It has been proven to have anti-inflammatory activity, but its specific anti-inflammatory components have not yet been fully elucidated. Tumor necrosis factor receptor-1 (TNFR1), which participates in important intracellular signaling pathways, mediates apoptosis, and functions as a regulator of inflammation, is often used as the target to develop anti-inflammatory drugs. The small peptides of snake venom have the advantages of weak immunogenicity and strong activity. To obtain the specific TNFR1 binding peptides, we constructed a T7 phage library of D. acutus venom glands, and then performed biopanning against TNFR1 on the constructed library. After biopanning three times, sev

SUBMITTER: Zhang K 

PROVIDER: S-EPMC8878721 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature