NMR Detection and Structural Modeling of the Ethylene Receptor LeETR2 from Tomato.

ABSTRACT: The gaseous plant hormone ethylene influences many physiological processes in plant growth and development. Plant ethylene responses are mediated by a family of ethylene receptors, in which the N-terminal transmembrane domains are responsible for ethylene binding and membrane localization. Until now, little structural information was available on the molecular mechanism of ethylene responses by the transmembrane binding domain of ethylene receptors. Here, we screened different constructs, fusion tags, detergents, and purification methods of the transmembrane sensor domain of ethylene receptors. However, due to their highly hydrophobic transmembrane domain (TMD), only a KSI-fused LeETR2_1-131 from tomato yielded a good-quality nuclear magnetic resonance (NMR) spectrum in the organ

SUBMITTER: Wei S 

PROVIDER: S-EPMC8879215 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature