Detection and Molecular Identification of Eight Candida Species in Clinical Samples by Simplex PCR.

ABSTRACT: Systemic candidiasis is a frequent opportunistic mycosis that can be life-threatening. Its main etiological agent is Candida albicans; however, the isolation of non-albicans Candida species has been increasing. Some of these species exhibit greater resistance to antifungals, so the rapid and specific identification of yeasts is crucial for a timely diagnosis and optimal treatment of patients. Multiple molecular assays have been developed, based mainly on polymerase chain reaction (PCR), showing high specificity and sensitivity to detect and identify Candida spp. Nevertheless, its application in diagnosis has been limited due to specialized infrastructure or methodological complexity. The objective of this study was to develop a PCR assay that detects and identifies some of the most common

SUBMITTER: Garcia-Salazar E 

PROVIDER: S-EPMC8880469 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature