Molecular organization and mechanics of single vimentin filaments revealed by super-resolution imaging.

ABSTRACT: Intermediate filaments (IFs) are involved in key cellular functions including polarization, migration, and protection against large deformations. These functions are related to their remarkable ability to extend without breaking, a capacity that should be determined by the molecular organization of subunits within filaments. However, this structure-mechanics relationship remains poorly understood at the molecular level. Here, using super-resolution microscopy (SRM), we show that vimentin filaments exhibit a ~49-nanometer axial repeat both in cells and in vitro. As unit-length filaments (ULFs) were measured at ~59 nanometers, this demonstrates a partial overlap of ULFs during filament assembly. Using an SRM-compatible stretching device, we also provide evidence that the extensibility of vim

SUBMITTER: Nunes Vicente F 

PROVIDER: S-EPMC8880768 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature