Multiplex Sensing of Complex Mixtures by Machine Vision Analysis of TLC-SERS Images.

ABSTRACT: Thin layer chromatography in tandem with surface-enhanced Raman scattering (TLC-SERS) has demonstrated tremendous potentials as a new analytical chemistry tool to detect a wide range of substances from real-world samples. However, it still faces significant challenges of multiplex sensing from complex mixtures due to the imperfect separation by TLC and the resulting interference of SERS detection. In this article, we propose a multiplex sensing method of complex mixtures by machine vision analysis of the scanning image of the TLC-SERS results. Briefly, various pure substances in solution and the complex mixture solution are separated by TLC followed by one-dimensional SERS scanning of the entire TLC plate, which generates TLC-SERS images of all target substances along the chromatography pa

SUBMITTER: Hou X 

PROVIDER: S-EPMC8880841 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature