Adaptation of the protein translational apparatus during ATDC5 chondrogenic differentiation.

ABSTRACT:

Introduction

Ribosome biogenesis is integrated with many cellular processes including proliferation, differentiation and oncogenic events. Chondrogenic proliferation and differentiation require a high cellular translational capacity to facilitate cartilaginous extracellular matrix production. We here investigated the expression dynamics of factors involved in ribosome biogenesis during in vitro chondrogenic differentiation and determined whether protein translation capacity adapts to different phases of chondrogenic differentiation.

Materials

SnoRNA expression during ATDC5 differentiation was analyzed by RNA sequencing of samples acquired from day 0 (progenitor stage), 7 (chondrogenic stage) and day 14 (hypertrophic stage). RT-qPCR was used to determine expression of

SUBMITTER: Steinbusch MMF 

PROVIDER: S-EPMC8881200 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature