The Origin and Contribution of Cancer-Associated Fibroblasts in Colorectal Carcinogenesis.

ABSTRACT:

Background & aims

Cancer-associated fibroblasts (CAFs) play an important role in colorectal cancer (CRC) progression and predict poor prognosis in CRC patients. However, the cellular origins of CAFs remain unknown, making it challenging to therapeutically target these cells. Here, we aimed to identify the origins and contribution of colorectal CAFs associated with poor prognosis.

Methods

To elucidate CAF origins, we used a colitis-associated CRC mouse model in 5 different fate-mapping mouse lines with 5-bromodeoxyuridine dosing. RNA sequencing of fluorescence-activated cell sorting-purified CRC CAFs was performed to identify a potential therapeutic target in CAFs. To examine the prognostic significance of the stromal target, CRC patient RNA sequencing data and tissue microar

SUBMITTER: Kobayashi H 

PROVIDER: S-EPMC8881386 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature