In vitro and in vivo functions of T cells produced in complemented thymi of chimeric mice generated by blastocyst complementation.

ABSTRACT: Blastocyst complementation is an intriguing way of generating humanized animals for organ preparation in regenerative medicine and establishing novel models for drug development. Confirming that complemented organs and cells work normally in chimeric animals is critical to demonstrating the feasibility of blastocyst complementation. Here, we generated thymus-complemented chimeric mice, assessed the efficacy of anti-PD-L1 antibody in tumor-bearing chimeric mice, and then investigated T-cell function. Thymus-complemented chimeric mice were generated by injecting C57BL/6 (B6) embryonic stem cells into Foxn1^nu/nu morulae or blastocysts. Flow cytometry data showed that the chimeric mouse thymic epithelial cells (TECs) were derived from the B6 cells. T cells appeared outside the thymi

SUBMITTER: Yamazaki K 

PROVIDER: S-EPMC8881621 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature