Small-volume in vitro lipid digestion measurements for assessing drug dissolution in lipid-based formulations using SAXS.

ABSTRACT: Lipid-based formulations improve the absorption capacity of poorly-water-soluble drugs and digestion of the formulation is a critical step in that absorption process. A recent approach to understanding the propensity for drug to dissolve in digesting lipid-based formulations couples an in vitro pH-stat lipolysis model to small-angle X-ray scattering (SAXS) by means of a flow-through capillary. However, the conventional pH-stat apparatus used to measure the extent of lipid digestion during such experiments requires digest volumes of 15-30 mL and drug doses of 50-200 mg, which is problematic for scarce compounds and can require excessive amounts of formulation reagents. This manuscript describes an approach to reduce the amount of material required for in vitro lipolysis experi

SUBMITTER: Khan NF 

PROVIDER: S-EPMC8881665 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature