The expression of essential selenoproteins during development requires SECIS-binding protein 2-like.

ABSTRACT: The dietary requirement for selenium is based on its incorporation into selenoproteins, which contain the amino acid selenocysteine (Sec). The Sec insertion sequence (SECIS) is an RNA structure found in the 3' UTR of all selenoprotein mRNAs, and it is required to convert in-frame UGA codons from termination to Sec-incorporating codons. SECIS-binding protein 2 (Sbp2) is required for Sec incorporation, but its paralogue, SECIS-binding protein 2-like (Secisbp2l), while conserved, has no known function. Here we determined the relative roles of Sbp2 and Secisbp2l by introducing CRISPR mutations in both genes in zebrafish. By monitoring selenoprotein synthesis with ⁷⁵Se labeling during embryogenesis, we found that sbp2 ^-/- embryos still make a select subset of

SUBMITTER: Kiledjian NT 

PROVIDER: S-EPMC8881744 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature