ICAM-1-mediated adhesion is a prerequisite for exosome-induced T cell suppression.

ABSTRACT: Tumor-derived extracellular vesicles (TEVs) suppress the proliferation and cytotoxicity of CD8⁺ T cells, thereby contributing to tumor immune evasion. Here, we report that the adhesion molecule intercellular adhesion molecule 1 (ICAM-1) co-localizes with programmed death ligand 1 (PD-L1) on the exosomes; both ICAM-1 and PD-L1 are upregulated by interferon-γ. Exosomal ICAM-1 interacts with LFA-1, which is upregulated in activated T cells. Blocking ICAM-1 on TEVs reduces the interaction of TEVs with CD8⁺ T cells and attenuates PD-L1-mediated suppressive effects of TEVs. During this study, we have established an extracellular vesicle-target cell interaction detection through SorTagging (ETIDS) system to assess the interaction between a TEV ligand and its target cell rece

SUBMITTER: Zhang W 

PROVIDER: S-EPMC8881799 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature